Ervatamin-C (Tabernaemontana divaricata)

Definition:

  synonymsAndTranslations

Ervatamin-C
Ervatamin-C (Tabernaemontana divaricata)
ERVC_TABDI
ERV-C edit

  functional

has function:
Cysteine proteinase. Hydrolyzes denatured natural substrates such as casein, hemoglobin, azoalbumin and azocasein with a high specific activity. Has little or no activity against synthetic substrates.

enzyme regulation by:
Activated by thio-specific activators such as cysteine, beta-mercaptoethanol, DTT and glutathione. Inhibited by the thiol-specific inhibitors leupeptin, iodoacetamide, PCMB, NEM, mercuric chloride and sodium tetrathionate.

performs:
EC 3.4.22.-

gene product has molecular function:
lysosomal cysteine-type endopeptidase
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  localization

gene product expressed in tissue:
Laticifer.

has subcellular localization:
Secreted.

occurs in:
Tabernaemontana divaricata
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  structural

component of:
Monomer.

has mass spectrometry data:
23000.0 is the determined molecular weight; MALDI is the ionization method; 1-208 is the part of the protein sequence entry that corresponds to the molecular weight;
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  pharmaceutical

has biophysicochemical properties:
Kinetic parameters:(KM=9.09 uM for azoalbumin; ); pH dependence: Optimum pH is 7.5-8.0 with azoalbumin or hemoglobin as substrate. Active and stable from pH 2.0 to 12.0; temperature dependence: Optimum temperature is 50 degrees Celsius with azoalbumin as substrate. Thermostable up to 70 degrees Celsius;
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  conceptual

gene product plays role in biological process:
Proteolysis

similarity confers association with:
peptidase C1 family
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  references

source:
Swiss-Prot
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  classMembership

amino acid, peptide, or protein
Ervatamin-C edit